Enzyme Experiment
 
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Student Results  2000                                              

                                                                                   
Baseline (determine amount of H2O2
 initially present in a 1.5% solution)                               
10 mL H2O2
 1 mL H2O
10 mL H2SO4

titrate 5 mL aliquot: 4.2 mL is baseline
Spontaneous Decomposition of H2O2
Amount of KMnO4 titrant :  3.9 mL
% spontaneous decomposition over 24 hours period:
(mL baseline - mL 24 hours)/mL baseline * 100 = 2.4% spontaneous decomposition
Timed Course Determination of Enzyme Catalyzed Decomposition of H2O2
KMnO4 mL  Time (sec)        
    10 30 60 120 180 360
Baseline
Final Reading Burette
Initial Reading Burette
Am't  KMnO4 consumed
Amount of H2O2   used
 4.2
15.2
12.5
2.7
1.5
 4.2
19
17.5
1.5
2.7
 4.2
19.9
19.2
0.7
3.5
 4.2
20.2
19.9
0.3
3.9
 4.2
20.3
20.2
0.1
4.1
 4.2
20.55
20.5
0.05
4.5
Rate of Catalyzed Decomposition Reaction for Successive Time Intervals
Time Intervals (sec)

10
20
30
60
60
180

 Rates mL/sec

0.15
1.35
0.117
0.065
0.068
0.023